Premium
The effects of fluorescent labels on Aβ 42 aggregation detected by fluorescence correlation spectroscopy
Author(s) -
Zheng Yanpeng,
Xu Lingwan,
Yang Jingfa,
Peng Xianglei,
Wang He,
Yu Na,
Hua Ying,
Zhao Jiang,
He Jinsheng,
Hong Tao
Publication year - 2018
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.23237
Subject(s) - fluorescence , chemistry , rhodamine , fluorescence correlation spectroscopy , fluorescein , fluorescein isothiocyanate , fluorescence spectroscopy , isothiocyanate , bodipy , rhodamine b , photochemistry , biophysics , molecule , organic chemistry , physics , quantum mechanics , photocatalysis , biology , catalysis
Fluorescence‐based methods are promising for measuring amyloid beta (Aβ) oligomers, given their capacity to analyse a sample at the single‐molecule level. As the attachment of fluorescent labels may influence the biochemical properties of the Aβ oligomers, the effects of fluorescent labels on Aβ oligomers must be evaluated. In this paper, we compared the impacts of five different fluorescent dyes on the aggregation of Aβ 42 oligomers using fluorescence correlation spectroscopy (FCS). We found that fluorescent labels of BODIPY® FL‐C5 (BP), N‐hydroxysuccinimide rhodamine B ester (RB) and rhodamine B isothiocyanate (RITC) increased the propensity of labelled Aβ 42 oligomers to aggregate, whereas 6‐(fluorescein‐5‐carboxamido) hexanoic acid succinimidyl ester (5‐SFX) and fluorescein 5(6)‐isothiocyanate (5(6)‐FITC) decreased the propensity of labelled Aβ 42 oligomers to aggregate. This difference originated from the different electric charges and hydrophobicity of the fluorescent dyes. These results provide valuable information for establishing different aggregation models for Aβ 42 oligomers in vitro using FCS.