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PHBV‐TiO 2 mats prepared by electrospinning technique: Physico‐chemical properties and cytocompatibility
Author(s) -
Braga Natália F.,
Vital Daniel A.,
Guerrini Lilia M.,
Lemes Ana P.,
Formaggio Daniela M. D.,
Tada Dayane B.,
Arantes Tatiane M.,
Cristovan Fernando H.
Publication year - 2018
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.23120
Subject(s) - electrospinning , thermogravimetric analysis , differential scanning calorimetry , nanocomposite , crystallization , biocompatibility , chemical engineering , nanoparticle , nucleation , chemistry , thermal stability , tissue engineering , scanning electron microscope , materials science , polymer , composite material , organic chemistry , biomedical engineering , medicine , physics , engineering , thermodynamics
Abstract One of the most important challenges in tissue engineering research is the development of biomimetic materials. In this present study, we have investigated the effect of the titanium dioxide (TiO 2 ) nanoparticles on the properties of electrospun mats of poly (hydroxybutyrate‐ co ‐3‐hydroxyvalerate) (PHBV), to be used as scaffold. The morphology of electrospun fibers was observed by scanning electron microscopy (SEM). Both pure PHBV and nanocomposites fibers were smooth and uniform. However, there was an increase in fiber diameter with the increase of TiO 2 concentration. Thermal properties of PHBV and nanocomposite mats were characterized by differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA). DSC analysis showed that the crystallization temperature for PHBV shifts to higher temperature in the presence of the nanoparticles, indicating that TiO 2 nanoparticles change the process of crystallization of PHBV due to heterogeneous nucleation effect. TGA showed that in the presence of the nanoparticles, the curves are shifted to lower temperatures indicating a decreasing in thermal stability of nanocomposites compared to pure PHBV. To produce scaffolds for tissue engineering, it is important to evaluate the biocompatibility of the material. Cytotoxicity assay showed that TiO 2 nanoparticles were not cytotoxic for cells at the concentration used to synthesize the mats. The proliferation of cells on the mats was evaluated by the MTT assay. Results showed that the nanocomposite samples increased cell proliferation compared to the pure PHBV. These results indicate that continuous electrospun fibrous scaffolds may be a good substrate for tissue regeneration.