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Synthetic polymers as substrates for a DNA‐sliding clamp protein
Author(s) -
van Dongen S. F. M.,
Clerx J.,
van den Boomen O. I.,
Pervaiz M.,
Trakselis M. A.,
Ritschel T.,
Schoonen L.,
Schoenmakers D. C.,
Nolte R. J. M.
Publication year - 2018
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.23119
Subject(s) - chemistry , dna polymerase , dna clamp , clamp , polymerase , polymer , dna , biophysics , computational biology , copying , nanotechnology , biochemistry , gene , polymerase chain reaction , genetics , biology , computer science , organic chemistry , reverse transcriptase , clamping , computer vision , materials science
The clamp protein (gp45) of the DNA polymerase III of the bacteriophage T4 is known to bind to DNA and stay attached to it in order to facilitate the process of DNA copying by the polymerase. As part of a project aimed at developing new biomimetic data‐encoding systems we have investigated the binding of gp45 to synthetic polymers, that is, rigid, helical polyisocyanopeptides. Molecular modelling studies suggest that the clamp protein may interact with the latter polymers. Experiments aimed at verifying these interactions are presented and discussed.