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Enzymatic degradation of hyaluronan hydrogels with different capacity for in situ bio‐mineralization
Author(s) -
Shi Liyang,
Zhang Yu,
Ossipov Dmitri
Publication year - 2018
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.23090
Subject(s) - self healing hydrogels , chemistry , mineralization (soil science) , degradation (telecommunications) , in situ , biomineralization , biophysics , covalent bond , chemical engineering , fluorescence , matrix (chemical analysis) , enzyme , polymer chemistry , biochemistry , chromatography , organic chemistry , nitrogen , telecommunications , physics , quantum mechanics , computer science , engineering , biology
In situ cross‐linked hyaluronan (HA) hydrogels with different capacities for biomineralization were prepared and their enzymatic degradation was monitored. Covalent incorporation of bisphosphonates (BPs) into HA hydrogel results in the increased stiffness of the hydrogel in comparison with the unmodified HA hydrogel of the same cross‐linking density. The rate of enzymatic degradation of HABP hydrogel was significantly lower than the rate of degradation of control HA hydrogel in vitro. This effect is observed only in the presence of calcium ions that strongly bind to the matrix‐anchored BP groups and promote further mineralization of the matrix. The degradation of the hydrogels was followed by noninvasive fluorescence measurements enabled after mild and chemoselective labeling of cross‐linkable HA derivatives with a fluorescent tag.