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Design of modular dual enzyme‐responsive peptides
Author(s) -
Boehnke Natalie,
Maynard Heather D.
Publication year - 2017
Publication title -
peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.23035
Subject(s) - chemistry , enzyme , peptide , amine gas treating , cleavage (geology) , lysine , biochemistry , modular design , combinatorial chemistry , stereochemistry , amino acid , organic chemistry , geotechnical engineering , fracture (geology) , computer science , engineering , operating system
Dual enzyme‐responsive peptides were synthesized by masking the ɛ‐amine of lysine with various enzyme substrates. Enzymatic cleavage of these sequences unmasked the ɛ‐amine, allowing for further digestion by a second enzyme, which was monitored colorimetrically. This modular peptide design should provide substrates for a large combination of clinically relevant enzymes.