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Identification of peptide sequences that selectively bind to pentaerythritol trinitrate hemisuccinate‐a surrogate of PETN, via phage display technology
Author(s) -
Kubas George,
Rees William,
Caguiat Jonathan,
Asch David,
Fagan Diana,
Cortes Pedro
Publication year - 2017
Publication title -
peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.22997
Subject(s) - pentaerythritol tetranitrate , chemistry , peptide , amino acid , peptide library , phage display , panning (audio) , pentaerythritol , combinatorial chemistry , biochemistry , peptide sequence , stereochemistry , explosive material , organic chemistry , gene , biology , paleontology , zoom , fire retardant , lens (geology)
The present research investigates the identification of amino acid sequences that selectively bind to a pentaerythritol tetranitrate (PETN) explosive surrogate. Through the use of a phage display technique and enzyme‐linked immunosorbent assays (ELISA), a peptide library was tested against pentaerythritol trinitrate hemisuccinate (PETNH), a surrogate of PETN, to screen for those with amino acids having affinity toward the explosive. The results suggest that the library contains peptides selective to PETNH. Following three rounds of panning, clones were picked and tested for specificity toward PETNH. ELISA results from these samples show that each phage clone has some level of selectivity for binding to PETNH. The peptides from these clones have been sequenced and shown to contain certain common amino acid segments among them. This work represents a technological platform for identifying amino‐acid sequences selective toward any bio‐chem analyte of interest.