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Silk scaffolds connected with different naturally occurring biomaterials for prostate cancer cell cultivation in 3 D
Author(s) -
Bäcker Anne,
Erhardt Olga,
Wietbrock Lukas,
Schel Natalia,
Göppert Bettina,
Dirschka Marian,
Abaffy Paul,
Sollich Thomas,
Cecilia Angelica,
Gruhl Friederike J.
Publication year - 2017
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.22993
Subject(s) - fibroin , chemistry , bombyx mori , lncap , silk , chitosan , scaffold , biopolymer , gelatin , swelling , polymer , chemical engineering , biomedical engineering , cancer cell , materials science , composite material , biochemistry , cancer , organic chemistry , biology , medicine , genetics , gene , engineering
In the present work, different biopolymer blend scaffolds based on the silk protein fibroin from Bombyx mori (BM) were prepared via freeze‐drying method. The chemical, structural, and mechanical properties of the three dimensional (3D) porous silk fibroin (SF) composite scaffolds of gelatin, collagen, and chitosan as well as SF from Antheraea pernyi (AP) and the recombinant spider silk protein spidroin (SSP1) have been systematically investigated, followed by cell culture experiments with epithelial prostate cancer cells (LNCaP) up to 14 days. Compared to the pure SF scaffold of BM, the blend scaffolds differ in porous morphology, elasticity, swelling behavior, and biochemical composition. The new composite scaffold with SSP1 showed an increased swelling degree and soft tissue like elastic properties. Whereas, in vitro cultivation of LNCaP cells demonstrated an increased growth behavior and spheroid formation within chitosan blended scaffolds based on its remarkable porosity, which supports nutrient supply matrix. Results of this study suggest that silk fibroin matrices are sufficient and certain SF composite scaffolds even improve 3D cell cultivation for prostate cancer research compared to matrices based on pure biomaterials or synthetic polymers.