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Biological activity of peptide‐conjugated polyion complex matrices consisting of alginate and chitosan
Author(s) -
Fujimori Chikara,
Kumai Jun,
Nakamura Kyotaro,
Gu Yingzi,
Katagiri Fumihiko,
Hozumi Kentaro,
Kikkawa Yamato,
Nomizu Motoyoshi
Publication year - 2017
Publication title -
peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.22983
Subject(s) - peptide , chemistry , laminin , chitosan , polysaccharide , biomaterial , conjugated system , tissue engineering , integrin , biochemistry , biophysics , combinatorial chemistry , extracellular matrix , cell , polymer , organic chemistry , biomedical engineering , medicine , biology
Peptide‐conjugated polysaccharide matrices using bioactive laminin‐derived peptides are useful biomaterials for tissue and cell engineering. Here, we demonstrate an easy handling preparation method for peptide‐polysaccharide matrices using polyion complex with both alginate and chitosan. First, aldehyde‐alginate was synthesized by oxidization of alginate using NaIO 4 , and then, reacted with Cys‐peptides. Next, the peptide‐alginate solution was added to a chitosan‐coated plate, and the peptide‐polyion complex matrices (peptide‐PCMs) were prepared. The peptide‐PCMs using an integrin αvβ3‐binding peptide (A99a: ALRGDN, mouse laminin α1 chain 1145‐1150) and an integrin α2β1‐binding peptide (EF1XmR: RLQLQEGRLHFXFD, X = Nle, mouse laminin α1 chain 2751‐2763) showed strong cell attachment activity in a dose‐dependent manner. When we examined the effect of various spacers on the biological activity of A99a‐PCM, hydrophobic and long spacers enhanced the cell attachment activity. Further, the A99a‐PCM with the spacers strongly promoted neurite outgrowth. The polyion complex method is an easy way to obtain insolubilized matrix and is widely applicable for various polysaccharides. The peptide‐PCM is useful as a biomaterial for cell and tissue engineering.