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Estimation of the diversity between DNA calorimetric profiles, differential melting curves and corresponding melting temperatures
Author(s) -
Chang ChunLing,
Fridman Alexander S.,
Grigoryan Inessa E.,
Galyuk Ele.,
Murashko Oleg N.,
Hu ChinKun,
Lando Dmitri Y.
Publication year - 2016
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.22918
Subject(s) - chemistry , melting curve analysis , melting temperature , differential (mechanical device) , differential scanning calorimetry , thermodynamics , dna , melting pot , diversity (politics) , polymerase chain reaction , biochemistry , composite material , gene , materials science , physics , history , archaeology , immigration , sociology , anthropology
The Poland–Fixman–Freire formalism was adapted for modeling of calorimetric DNA melting profiles, and applied to plasmid pBR 322 and long random sequences. We studied the influence of the difference ( H GC − H AT ) between the helix‐coil transition enthalpies of AT and GC base pairs on the calorimetric melting profile and on normalized calorimetric melting profile. A strong alteration of DNA calorimetrical profile with H GC − H AT was demonstrated. In contrast, there is a relatively slight change in the normalized profiles and in corresponding ordinary (optical) normalized differential melting curves (DMCs). For fixed H GC − H AT , the average relative deviation ( S ) between DMC and normalized calorimetric profile, and the difference between their melting temperatures ( T cal − T m ) are weakly dependent on peculiarities of the multipeak fine structure of DMCs. At the same time, both the deviation S and difference ( T cal − T m ) enlarge with the temperature melting range of the helix‐coil transition. It is shown that the local deviation between DMC and normalized calorimetric profile increases in regions of narrow peaks distant from the melting temperature.