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Method for generation of peptide‐specific igy antibodies directed to Staphylococcus aureus extracellular fibrinogen binding protein epitope
Author(s) -
Walczak Maciej,
Grzywa Renata,
ŁupickaSłowik Agnieszka,
Skoreński Marcin,
Bobrek Kamila,
Nowak Daria,
Boivin Stephane,
Brown Eric L.,
Oleksyszyn Józef,
Sieńczyk Marcin
Publication year - 2015
Publication title -
peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.22695
Subject(s) - epitope , staphylococcus aureus , chemistry , antibody , avidity , linear epitope , microbiology and biotechnology , fibrinogen , antigen , protein a , biochemistry , bacteria , biology , immunology , genetics
The IgY antibodies offer an attractive alternative to mammalian IgGs in research, diagnosis and medicine. The isolation of immunoglobulin Y from the egg yolks is efficient and economical, causing minimal suffering to animals. Here we present the methodology for the production of IgY antibodies specific to Staphylococcus aureus fibrinogen binding protein (Efb) and its peptidyl epitope (spanning residues 127–140). The Efb is an extracellular, adhesion protein which binds both human fibrinogen and complement C3 protein thus contributing to the high infectious potential of this pathogen. The selected epitope of Efb protein is responsible for the interaction with C3. The immunochemical characterization of both anti‐Efb and epitope‐specific IgY antibodies revealed their similar avidity, titer, and reactivity profile, although some differences in the hen's immune response to administered antigens is discussed. © 2015 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 104: 552–559, 2015.