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Modification of the degree of branching of a beta‐(1,3)‐glucan affects aggregation behavior and activity in an oxidative burst assay
Author(s) -
Magee Andrew S.,
Langeslay Ryan R.,
Will Paul M.,
Danielson Michael E.,
Wurst Lindsay R.,
Iiams Vanessa A.
Publication year - 2015
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.22685
Subject(s) - chemistry , branching (polymer chemistry) , oxidative phosphorylation , beta (programming language) , beta glucan , glucan , biophysics , biochemistry , organic chemistry , computer science , biology , programming language
Scleroglucan is a β‐(1,3)‐glucan which is highly branched at the 6‐position with a single glucose residue. Acid hydrolysis of a high molecular weight scleroglucan gave a medium molecular weight, freely soluble material. Linkage analysis by the partially methylated alditol acetate method showed that the solubilized material had 30% branching. When the material was subjected to partial Smith degradations, the percent branching was reduced accordingly to 12% or 17%. After the percent branching was reduced, the average molecular weight of the samples increased considerably, indicating the assembly of higher ordered aggregate structures. An aggregate number distribution analysis was applied to confirm the higher aggregated structures. These aggregated structures gave the material significantly enhanced activity in an in vitro oxidative burst assay compared to the highly branched material. © 2015 Wiley Periodicals, Inc. Biopolymers 103: 665–674, 2015.