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A highly sensitive square wave voltammetry based biosensor for kinase activity measurements
Author(s) -
Snir Elza,
Amit Einav,
Friedler Assaf,
Yitzchaik Shlomo
Publication year - 2015
Publication title -
peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.22653
Subject(s) - chemistry , square wave , monolayer , biosensor , electrode , analytical chemistry (journal) , cyclic voltammetry , electrochemistry , peptide , dynamic range , voltammetry , chromatography , biochemistry , optics , physics , quantum mechanics , voltage
An electrochemical biosensor has been developed for ultrasensitive, label‐free determination of protein kinase activity. The sensor is composed of a unique peptide monolayer on a gold electrode. It identifies the order change in the monolayer upon phosphorylation, via square wave voltametry (SWV) measurements. Disorder caused by the introduction of the phosphate groups onto the middle of the peptide sequence results in pinhole formation and therefore an increase in the electrochemical signal. The measured sensitivity was 100 n M of kinase and the dynamic range was 100 n M up to 11 μ M . Sensitivity was an order of magnitude higher, and the dynamic range wider by two orders of magnitude, as compared to our previously reported impedimetric method, in which the sensitivity was 1 μ M , and the dynamic range was 1–20 μ M . © 2015 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 104: 515–520, 2015.