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Solid‐phase synthesis, characterization, and cellular activities of collagen‐model Nanodiamond‐peptide conjugates
Author(s) -
Knapinska Anna M.,
TokminaRoszyk Dorota,
Amar Sabrina,
TokminaRoszyk Michal,
Mochalin Vadym N.,
Gogotsi Yury,
Cosme Patrick,
Terentis Andrew C.,
Fields Gregg B.
Publication year - 2015
Publication title -
peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.22636
Subject(s) - biomolecule , chemistry , nanodiamond , peptide , solid phase synthesis , covalent bond , conjugate , conjugated system , combinatorial chemistry , in vitro , in vivo , drug delivery , nanotechnology , bioconjugation , biophysics , cell adhesion , biochemistry , cell , organic chemistry , polymer , materials science , mathematical analysis , mathematics , microbiology and biotechnology , diamond , biology
Nanodiamonds (NDs) have received considerable attention as potential drug delivery vehicles. NDs are small (∼5 nm diameter), can be surface modified in a controllable fashion with a variety of functional groups, and have little observed toxicity in vitro and in vivo . However, most biomedical applications of NDs utilize surface adsorption of biomolecules, as opposed to covalent attachment. Covalent modification provides reliable and reproducible ND–biomolecule ratios, and alleviates concerns over biomolecule desorption prior to delivery. The present study has outlined methods for the efficient solid‐phase conjugation of ND to peptides and characterization of ND–peptide conjugates. Utilizing collagen‐derived peptides, the ND was found to support or even enhance the cell adhesion and viability activities of the conjugated sequence. Thus, NDs can be incorporated into peptides and proteins in a selective manner, where the presence of the ND could potentially enhance the in vivo activities of the biomolecule it is attached to. © 2015 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 104: 186–195, 2015.