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Hydrolysis of pullulan by entrapped pullulanase in Ca/alginate beads
Author(s) -
Gasmi Sarah Nawel,
Rihouey Christophe,
Picton Luc,
Cerf Didier
Publication year - 2014
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.22485
Subject(s) - pullulan , pullulanase , chemistry , maltotriose , hydrolysis , chromatography , substrate (aquarium) , polysaccharide , enzyme , organic chemistry , maltose , oceanography , geology
Pullulanase from Klebsiella pneumoniae was entrapped into calcium alginate beads. Its activity was estimated by the determination of number–average molar masses using two different methods: a colorimetric assay of reducing ends (REs) and a size‐exclusion chromatography/multiangle light scattering/differential refractive index. The second method also provided weight–average molar masses of hydrolyzed pullulan and the quantity of maltotriose (DP3) and its multiples (DP6 and DP9) produced by the enzymatic treatment. The alginate beads showed a good retention of the loaded pullulanase (30%), and the system showed a downturn of hydrolysis kinetics in comparison with free pullulanase due to the limiting access of substrate–enzyme. On the contrary with the results obtained from free enzyme hydrolysis, for which a large distribution of pullulan fragments is observed during the treatment, the immobilized enzyme system has evidenced, during the enzymatic treatment, the coexistence of native or only slightly degraded pullulan chains together with maltotriose units. Complete hydrolysis of pullulan chains was achieved once diffused into the gel. © 2014 Wiley Periodicals, Inc. Biopolymers 101: 938–944, 2014.