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A personal perspective on chemistry‐driven RNA research
Author(s) -
Micura Ronald,
Kreutz Christoph,
Breuker Kathrin
Publication year - 2013
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.22299
Subject(s) - chemistry , rna , riboswitch , fourier transform ion cyclotron resonance , mass spectrometry , förster resonance energy transfer , computational biology , biophysics , biochemistry , non coding rna , fluorescence , chromatography , physics , biology , gene , quantum mechanics
In this mini review, we discuss how our understanding of ribonucleic acid (RNA) properties becomes significantly deepened when a broad range of modern chemical and biophysical methods is applied. We span our perspective from RNA solid‐phase synthesis and site‐specific labeling to single‐molecule fluorescence‐resonance‐energy‐transfer imaging and NMR spectroscopy approaches to explore the dynamics of RNA over a broad timescale. We then move on to Fourier‐transform‐ion‐cyclotron‐resonance mass spectrometry (FT‐ICR‐MS) as a powerful technique for RNA sequencing and modification analysis. The novel methodological developments are discussed for selected biological systems that include the thiamine‐pyrophosphate riboswitch, HIV and ribosomal A‐site RNA, and transfer RNA. © 2013 Wiley Periodicals, Inc. Biopolymers 99: 1114–1123, 2013.