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Influence of poly( n ‐isopropylacrylamide)–CNT–polyaniline three‐dimensional electrospun microfabric scaffolds on cell growth and viability
Author(s) -
Tiwari Ashutosh,
Sharma Yashpal,
Hattori Shinya,
Terada Dohiko,
Sharma Ashok K.,
Turner Anthony P. F.,
Kobayashi Hisatoshi
Publication year - 2013
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.22170
Subject(s) - polyaniline , poly(n isopropylacrylamide) , chemistry , chemical engineering , electrospinning , polymer chemistry , nanotechnology , materials science , copolymer , polymer , organic chemistry , polymerization , engineering
This study investigates the effect on: (1) the bulk surface and (2) the three‐dimensional non‐woven microfabric scaffolds of poly(N‐isopropylacrylamide)–CNT–polyaniline on growth and viability of cells. The poly(N‐isopropylacrylamide)–CNT–polyaniline was prepared using coupling chemistry and electrospinning was then used for the fabrication of responsive, non‐woven microfabric scaffolds. The electrospun microfabrics were assembled in regular three‐dimensional scaffolds with OD: 400–500 μm; L: 6–20 cm. Mice fibroblast cells L929 were seeded on the both poly(N‐isopropylacrylamide)–CNT–polyaniline bulk surface as well as non‐woven microfabric scaffolds. Excellent cell proliferation and viability was observed on poly(N‐isopropylacrylamide)–CNT–polyaniline non‐woven microfabric matrices in compare to poly(N‐isopropylacrylamide)–CNT–polyaniline bulk and commercially available Matrigel™ even with a range of cell lines up to 168 h. Temperature dependent cells detachment behavior was observed on the poly(N‐isopropylacrylamide)–CNT–polyaniline scaffolds by varying incubation at below lower critical solution temperature of poly(N‐isopropylacrylamide). The results suggest that poly(N‐isopropylacrylamide)–CNT–polyaniline non‐woven microfabrics could be used as a smart matrices for applications in tissue engineering. © 2012 Wiley Periodicals, Inc. Biopolymers 99: 334–341, 2013.