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Quadruplexes of human telomere DNA analogs designed to contain G:A:G:A, G:G:A:A, and A:A:A:A tetrads
Author(s) -
Sagi Janos,
Renčiuk Daniel,
Tomaško Martin,
Vorlíčková Michaela
Publication year - 2010
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.21481
Subject(s) - telomere , chemistry , g quadruplex , dna , computational biology , stereochemistry , combinatorial chemistry , nanotechnology , biochemistry , biology , materials science
Replacement of two to four guanines by adenines in the human telomere DNA repeat dG 3 (TTAG 3 ) 3 did not hinder the formation of quadruplexes if the substitutions took place in the terminal tetrad bridged by the diagonal loop of the intramolecular antiparallel three‐tetrad scaffold, as proved by CD and PAGE in both Na + and K + solutions. Thermodynamic data showed that, in Na + solution, the dG 3 (TTAG 3 ) 3 quadruplex was destabilized, the least by the two G:A:G:A tetrads, the most by the G:G:A:A tetrad in which the adenosines replaced syn ‐guanosines. In physiological K + solution, the highest destabilization was caused by the 4A tetrad. In K + , only the unmodified dG 3 (TTAG 3 ) 3 quadruplex rearranged into a K + ‐dependent quadruplex form, none of the multiple adenine‐modified structures did so. This may imply biological consequences for nonrepaired A‐for‐G mutations. © 2010 Wiley Periodicals, Inc. Biopolymers 93: 880–886, 2010.