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Biological activity of laminin peptide‐conjugated alginate and chitosan matrices
Author(s) -
Yamada Yuji,
Hozumi Kentaro,
Katagiri Fumihiko,
Kikkawa Yamato,
Nomizu Motoyoshi
Publication year - 2010
Publication title -
peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.21429
Subject(s) - chemistry , laminin , neurite , peptide , chitosan , integrin , tissue engineering , extracellular matrix , biological activity , biophysics , biochemistry , polysaccharide , cell , microbiology and biotechnology , in vitro , biology , genetics
Laminin active peptide‐conjugated chitosan mambranes have been previously demonstrated as a useful biomaterial for tissue engineering. Here, three laminin active peptides, A99 (AGTFALRGDNPQG), AG73 (RKRLQVQLSIRT), and EF1zz (ATLQLQEGRLHFXFDLGKGR, X: Nle), which interact with integrin αvβ3, syndecans, and integrin α2β1, respectively, were conjugated to alginate and evaluated the biological activities. A99‐alginate (3–3000 ng/mm 2 ) promoted cell attachment depending on the amount of alginate. More than 300 ng/mm 2 of the A99‐alginate matrices effectively promoted cell attachment, cell spreading with well‐organized actin stress fibers, and neurite outgrowth. AG73‐ and EF1zz‐alginates promoted strong cell attachment at the all amounts (3–3000 ng/mm 2 ). A99‐alginate (30–3000 ng/mm 2 ) promoted strong neurite outgrowth but lower amounts of A99‐alginate (3 ng/mm 2 ) showed weak activity. In contrast, AG73‐alginates (3–30 ng/mm 2 ) showed strong neurite outgrowth activity but higher amounts of AG73‐alginate (300–3000 ng/mm 2 ) decreased the activity. These data indicate that neurite outgrowth activity of peptide‐alginate matrices is peptide specific and the activity is dependent on the amount of alginate. Further, biological activities of the peptides on alginate and chitosan matrices were different, suggesting that the integrin‐ and syndecan‐mediated cellular functions on the peptide‐matrices are highly influenced by the scaffold structure including polysaccharide types and amounts. The laminin active peptide‐conjugated alginate and chitosan matrices can control receptor type specific functions and are useful for tissue engineering. © 2010 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 94: 711–720, 2010. This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com