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Exploiting biocatalysis in peptide self‐assembly
Author(s) -
Williams Richard J.,
Mart Robert J.,
Ulijn Rein V.
Publication year - 2010
Publication title -
peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.21346
Subject(s) - supramolecular chemistry , biocatalysis , nanotechnology , chemistry , nanomaterials , nanolithography , molecular recognition , kinetic control , self assembly , design elements and principles , biochemical engineering , catalysis , materials science , molecule , computer science , organic chemistry , reaction mechanism , medicine , alternative medicine , software engineering , pathology , fabrication , engineering
This review article covers recent developments in the use of enzyme‐catalyzed reactions to control molecular self‐assembly (SA), an area that merges the advantages of biocatalysis with soft materials self‐assembly. This approach is attractive because it combines biological (chemo‐, regio‐, and enantio‐) selectivity with the versatility of bottom up nanofabrication through dynamic SA. We define enzyme‐assisted SA (e‐SA) as the production of molecular building blocks from nonassembling precursors via enzymatic catalysis, where molecular building blocks form ordered structures via noncovalent interactions. The molecular design of SA precursors is discussed in terms of three key components related to (i) enzyme recognition, (ii) molecular switching mechanisms, and (iii) supramolecular interactions that underpin SA. This is followed by a discussion of a number of unique features of these systems, including spatiotemporal control of nucleation and structure growth, the possibility of controlling mechanical properties and the defect correcting and component selecting capabilities of systems that operate under thermodynamic control. Applications in biomedicine (biosensing, controlled release, matrices for wound healing, controlling cell fate by gelation) and bio(nano)technology (biocatalysts immobilization, nanofabrication, templating, and intracellular imaging) are discussed. Overall, e‐SA allows for unprecedented control over SA processes and provides a step forward toward production of nanostructures of higher complexity and with fewer defects as desired for next generation nanomaterials. © 2010 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 94:107–117, 2010. This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com