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C‐type natriuretic peptide: Structural studies, fragment synthesis, and preliminary biological evaluation in human osteosarcoma cell lines
Author(s) -
Shaikh Nasrin,
Russo Laura,
Papaleo Elena,
Giani Paolo,
De Gioia Luca,
Nicotra Francesco,
Quarto Rodolfo,
Cipolla Laura
Publication year - 2010
Publication title -
peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.21336
Subject(s) - chemistry , paracrine signalling , natriuretic peptide , peptide , cell growth , cell culture , biological activity , cell , biochemistry , hormone , endocrinology , medicine , microbiology and biotechnology , biology , receptor , in vitro , genetics , heart failure
Natriuretic peptides (NP) are a family of structurally related but genetically distinct hormones/paracrine factors that regulate blood volume, blood pressure, ventricular hypertrophy, pulmonary hypertension, fat metabolism, and long bone growth. In this work we present computational structural analysis of the three human NP in solution, the synthesis and preliminary biological assays of a short fragment of CNP, I 14 GSM 17 , together with one small mimetic, GGSM. Synthetic peptides IGSM, GGSM, and full length CNP were preliminary tested for their ability to influence cell growth of three human osteosarcoma cell lines. Synthetic peptides were shown to successfully mimic the biological activity of the full length natural peptide: their effects, although different upon the cell types used, are in accordance with the current literature, designating a possible role for CNP, and its derivatives, in skeletogenesis. © 2010 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 94: 213–219, 2010.