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N‐terminal truncated pyroglutamyl β amyloid peptide Aβ py 3‐42 shows a faster aggregation kinetics than the full‐length Aβ1‐42
Author(s) -
D'Arrigo Cristina,
Tabaton Massimo,
Perico Angelo
Publication year - 2009
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.21271
Subject(s) - chemistry , kinetics , peptide , circular dichroism , fibrillogenesis , fibril , biophysics , phosphate buffered saline , amyloid (mycology) , thioflavin , crystallography , biochemistry , alzheimer's disease , chromatography , disease , inorganic chemistry , physics , quantum mechanics , biology , medicine , pathology
We tested directly the differences in the aggregation kinetics of three important β amyloid peptides, the full‐length Aβ1‐42, and the two N‐terminal truncated and pyroglutamil modified Aβ py 3‐42 and Aβ py 11‐42 found in different relative concentrations in the brains in normal aging and in Alzheimer disease. By following the circular dichroism signal and the ThT fluorescence of the solution in phosphate buffer, we found substantially faster aggregation kinetics for Aβ py 3‐42. This behavior is due to the particular sequence of this peptide, which is also responsible for the specific oligomeric aggregation states, found by TEM, during the fibrillization process, which are very different from those of Aβ1‐42, more prone to fibril formation. In addition, Aβ py 3‐42 is found here to have an inhibitory effect on Aβ1‐42 fibrillogenesis, coherently with its known greater infective power. This is an indication of the important role of this peptide in the aggregation process of β‐peptides in Alzheimer disease. © 2009 Wiley Periodicals, Inc. Biopolymers 91: 861–873, 2009. This article was originally published online as an accepted preprint. The “Published Online“ date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com