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The immediate‐early oncoproteins Fra‐1, c‐Fos, and c‐Jun have distinguishable surface behavior and interactions with phospholipids
Author(s) -
Gaggiotti María Cecilia,
Del Boca Maximiliano,
Castro Gonzalo,
Caputto Beatriz L.,
Borioli Graciela A.
Publication year - 2009
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.21212
Subject(s) - chemistry , preprint , phospholipid , transcription factor , transcription (linguistics) , membrane , biophysics , computational biology , biochemistry , gene , biology , physics , linguistics , philosophy , quantum mechanics
This work explores the surface properties of the transcription factor Fra‐1 and compares them with those of two other immediate early proteins, c‐Fos and c‐Jun, to establish generalities and differences in the surface behavior and interaction with phospholipids of this type of proteins. We present several experimental clues of the flexible nature of Fra‐1, c‐Fos, and c‐Jun that support sequence‐based predictions of their intrinsical disorder. The values of surface parameters for Fra‐1 are similar in general to those of c‐Fos and c‐Jun. However, we find differences in the interactions of the three proteins with phospholipids. The closely related Fra‐1 and c‐Fos share affinity for anionic lipids but the former has more affinity for a condensed phase and senses a change in DPPC phase, while the latter has more affinity for an expanded phase. These features are in contrast with our previous finding that c‐Jun is not selective for phospholipid polar head group or charge. We show here that at least some immediate early transcription factors can interact with membrane phospholipids in a distinguishable manner, and this shall provide a basis for their potential capacity to regulate membrane‐mediated cellular processes. © 2009 Wiley Periodicals, Inc. Biopolymers 91: 710–718, 2009. This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com