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Development of a chitosan‐based nanoparticle formulation for delivery of a hydrophilic hexapeptide, dalargin
Author(s) -
Chen Yan,
Siddalingappa Basavaraj,
Chan Phoebe H. H.,
Benson Heather A. E.
Publication year - 2008
Publication title -
peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.21055
Subject(s) - nanoparticle , chitosan , chemistry , coacervate , zeta potential , particle size , chemical engineering , nanotechnology , cyclodextrin , nuclear chemistry , chromatography , organic chemistry , materials science , engineering
Nanoparticle based delivery systems can offer opportunities for targeting, controlled release, and enhanced stability of their drug, protein, or gene therapy payload. This study investigated the use of chitosan in combination with the ionic additives sulfobutyl‐ether‐7‐β‐cyclodextrin (SB‐CD) or SB‐CD/dextran sulfate (SB‐CD/DS) mixture in comparison with chitosan: DS in the formulation of nanoparticles incorporating the hexapeptide dalargin. The physical characteristics (particle size, zeta potential), entrapment and loading efficiency, and release of dalargin were quantified. It was demonstrated that anionic cyclodextrin, SB‐CD, can be used in complex coacervation with chitosan, with and without the presence of DS, to form nanoparticles. The presence of SB‐CD or DS in the nanoparticle formulation and the weight ratio of chitosan to anionic additive(s) influenced the physical properties of the nanoparticles and their ability to carry dalargin. In addition, the particle size of nanoparticles was also affected by the molecular weight of chitosan and DS. The use of either DS or SB‐CD/DS mixture produced chitosan nanoparticles with small particle size, high dalargin entrapment efficiency, enhanced peptide stability, and sustained release characteristics. © 2008 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 90: 663–670, 2008. This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com