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Structure and chain conformation of β‐glucan isolated from Auricularia auricula‐judae
Author(s) -
Ma Zhaocheng,
Wang Jianguo,
Zhang Lina
Publication year - 2008
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.20971
Subject(s) - chemistry , glucuronic acid , aqueous solution , intrinsic viscosity , polysaccharide , chromatography , size exclusion chromatography , molar mass distribution , extraction (chemistry) , analytical chemistry (journal) , organic chemistry , polymer , enzyme
From Auricularia auricula‐judae, a water soluble β‐ D ‐glucan, named as AAG, was isolated by extraction with 70% ethanol/water solution. Its chemical structure was analyzed by gas chromatography (GC), gas chromatography‐mass spectrometry (GC‐MS), matrix‐assisted laser desorption /ionization (MALDI)‐time of flight (TOF), and 1D, 2D NMR. AAG was detected, for the first time, to be composed of a main chain of (1→4)‐linked D ‐glucopyranosyl with glucopyranosyl side groups at O6. With the help of MALDI‐TOF‐MS, the sequence and the distribution of glucuronic acid were determined and the content of glucuronic acid is about 19%. Five fractions were prepared from the AAG sample in water by ultrasonic degradation method. Their molecular weight, size, and shape (chain conformation) were studied by dynamics light scattering (DLS), static laser light scattering (LLS), size exclusion chromatography combined LLS (SEC‐LLS) and viscometry in 0.1M NaCl aqueous solution at 25°C. The dependence of intrinsic viscosity ([η]) on M w for this polysaccharide was established to be [η] = 1.22 ×10 −3 M w 1.00 (cm 3 g −1 ) in the range of M w from 3.40 × 10 4 to 2.88 × 10 5 . The conformational parameters of the AAG polysaccharide were found to be 820 nm −1 for molar mass per unit contour length ( M L ), 12.3 nm for persistence length ( q ) and 2.1 for ρ (〈 s 2 〉 1/2 / R h ). The results suggested that the polysaccharide exists as extended chains in 0.1M NaCl aqueous solution. The chemical structure of AAG containing glucuronic acid and side groups led to steric hindrance, resulting in the increased stiffness of the chains. © 2008 Wiley Periodicals, Inc. Biopolymers 89: 614–622, 2008. This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com