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Ferrocenyl glycopeptides as electrochemical probes to detect autoantibodies in multiple sclerosis patients' sera
Author(s) -
RealFernández Feliciana,
Colson Amélie,
Bayardon Jérome,
Nuti Francesca,
Peroni Elisa,
MeunierPrest Rita,
Lolli Francesco,
Chelli Mario,
Darcel Christophe,
Jugé Sylvain,
Papini Anna Maria
Publication year - 2008
Publication title -
peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.20955
Subject(s) - autoantibody , glycopeptide , chemistry , electrochemistry , ferrocene , cyclic voltammetry , moiety , combinatorial chemistry , biosensor , amino acid , antibody , biochemistry , electrode , stereochemistry , immunology , medicine , antibiotics
Glycopeptide analogues of CSF114(Glc), modified at N‐terminus with new ferrocenyl carboxylic acid and a new ferrocenyl‐thiphosphino amino acid, were used to implement a new electrochemical biosensor for autoantibody detection in multiple sclerosis. The ferrocenyl moiety of these “electrochemical probes” did not affect autoantibody recognition both in SP‐ELISA and in inhibition experiments. By electrochemical monitoring the interactions of the modified peptides Fc‐CSF114(Glc) and 4‐FcPhP(S)Abu‐CSF114(Glc) with the autoantibodies, we demonstrated that autoantibodies could be detected with a sensitivity comparable to ELISA method. The new electrochemical probes can be proposed to characterize autoantibodies as biomarkers of multiple sclerosis by a simple, rapid, and reproducible cyclic voltammetry‐based diagnostic methodology. © 2008 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 90: 488–495, 2008. This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com