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Force spectroscopy of the fibrin(ogen)–fibrinogen interaction
Author(s) -
Chtcheglova Lilia A.,
Haeberli André,
Dietler Giovanni
Publication year - 2008
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.20910
Subject(s) - fibrin , fibrinogen , chemistry , coagulation , force spectroscopy , atomic force microscopy , hemostasis , spectroscopy , biophysics , thrombosis , nanotechnology , biochemistry , molecule , medicine , immunology , materials science , organic chemistry , physics , quantum mechanics , biology
Fibrin aggregation is of vital importance in many physiological and pathological processes, such as blood coagulation, wound healing, and thrombosis. In the present study, we investigated the forces involved in the initial steps of the fibrinogen fibrin aggregation by force spectroscopy using the atomic force microscope. Our data confirm the existence of strong specific interactions between fibrin and fibrin(ogen), with unbinding forces ranging from 290 to 375 pN and a logarithmic dependence on the loading rate between 0.8 and 23 nN/s. © 2007 Wiley Periodicals, Inc. Biopolymers 89: 292–301, 2008. This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com