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Time‐resolved fluorescence spectroscopy and molecular dynamics simulations point out the effects of pressure on the stability and dynamics of the porcine odorant‐binding protein
Author(s) -
Staiano Maria,
Saviano Michele,
Herman Petr,
Grycznyski Zygmunt,
Fini Carlo,
Varriale Antonio,
Parracino Antonietta,
Kold Ane Bisgaard,
Rossi Mosè,
D'Auria Sabato
Publication year - 2008
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.20908
Subject(s) - chemistry , hydrostatic pressure , molecular dynamics , preprint , fluorescence , dynamics (music) , fluorescence spectroscopy , biophysics , molecule , hydrostatic equilibrium , force spectroscopy , ligand (biochemistry) , spectroscopy , chemical physics , computational chemistry , biochemistry , thermodynamics , organic chemistry , receptor , physics , quantum mechanics , biology , world wide web , computer science , acoustics
The effects of hydrostatic pressure on the structure and stability of porcine odorant‐binding protein (pOBP) in the presence and absence of the odorant molecule 2‐isobutyl‐3‐methoxypyrazine (IBMP) were studied by steady‐state and time‐resolved fluorescence spectroscopy as well as by molecular dynamics simulation. The authors found that the application of moderate values of hydrostatic pressure to pOBP solutions perturbed the microenvironment of Trp 16 and disrupted its highly quenched complex with Met 39 . In addition, compared with the protein in the absence of IBMP, the MD simulations experiments carried out at different pressures highlighted the role of this ligand in stabilizing the Trp 16 /Met 39 interaction even at 2000 bar. The obtained results will assist for the tailoring of this protein as specific sensing element in a new class of fluorescence‐based biosensors for the detection of explosives. © 2007 Wiley Periodicals, Inc. Biopolymers 89: 284–291, 2008. This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com