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Review fluorescence correlation spectroscopy for probing the kinetics and mechanisms of DNA hairpin formation
Author(s) -
Orden Alan Van,
Jung Jaemyeong
Publication year - 2008
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.20826
Subject(s) - chemistry , fluorescence correlation spectroscopy , spectroscopy , fluorescence , fluorescence spectroscopy , dna , folding (dsp implementation) , nucleic acid , kinetics , photochemistry , chemical physics , molecule , biochemistry , physics , organic chemistry , quantum mechanics , electrical engineering , engineering
This article reviews the application of fluorescence correlation spectroscopy (FCS) and related techniques to the study of nucleic acid hairpin conformational fluctuations in free aqueous solutions. Complimentary results obtained using laser‐induced temperature jump spectroscopy, single‐molecule fluorescence spectroscopy, optical trapping, and biophysical theory are also discussed. The studies cited reveal that DNA and RNA hairpin folding occurs by way of a complicated reaction mechanism involving long‐ and short‐lived reaction intermediates. Reactions occurring on the subnanoseconds to seconds time scale have been observed, pointing out the need for experimental techniques capable of probing a broad range of reaction times in the study of such complex, multistate reactions. © 2007 Wiley Periodicals, Inc. Biopolymers 89: 1–16, 2008. This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com