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Fasciola gigantica cathepsin L proteinase‐based synthetic peptide for immunodiagnosis and prevention of sheep fasciolosis
Author(s) -
Jezek Jan,
Ridi Rashika El,
Salah Mohamed,
Wagih Amal,
Aziz Haidy W.,
Tallima Hatem,
El Shafie Mohamed H.,
Khalek Tarek Abdel,
Abo Ammou Faten F.,
Strongylis Constantinos,
Moussis Vassilios,
Tsikaris Vassilios
Publication year - 2007
Publication title -
peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.20788
Subject(s) - fasciolosis , fasciola gigantica , fasciola , chemistry , peptide , cathepsin , cathepsin l , biochemistry , microbiology and biotechnology , onchocerca volvulus , enzyme , biology , fasciola hepatica , immunology , helminths , onchocerciasis
Sheep fasciolosis is a devastating burden for the livestock industry. We herein report on immunodiagnosis of fasciolosis, and significant protection of sheep against challenge infection with Fasciola gigantica following immunization with a peptide based on the H‐Asp 110 ‐Lys‐Ile‐Asp‐Trp‐Arg‐Glu‐Ser‐Gly‐Tyr‐Val‐Thr‐Glu‐Val 123 ‐OH (Fas14p) sequence of F. gigantica cathepsin L‐cysteine proteinase. This sequence was synthesized in three different forms: as N α acetylated (Ac‐Asp 110 ‐Lys‐Ile‐Asp‐Trp‐Arg‐Glu‐Ser‐Gly‐Tyr‐Val‐Thr‐Glu‐Val 123 ‐OH, FasAc14p), bearing at the amino‐terminus an N α acetylated cystein (Ac‐Cys‐Asp 110 ‐Lys‐Ile‐Asp‐Trp‐Arg‐Glu‐Ser‐Gly‐Tyr‐Val‐Thr‐Glu‐Val 123 ‐OH, FasAcCys14p), and conjugated to sequential oligopeptide carrier Ac‐[Lys‐Aib‐Gly] 4 ‐OH (Ac‐SOC 4 ) through an amide bond formed between Val 123 carboxylic group of the epitope and the lysine N ϵ groups of the carrier (Ac‐[Lys(Fas14p)‐Aib‐Gly] 4 ‐OH). Ac‐[Lys(Fas14p)‐Aib‐Gly] 4 ‐OH failed to readily discriminate between naïve and infected sheep. In contrast, the free peptides reproducibly differentiated between parasite‐free sheep, sheep infected with parasites other than Fasciola , and experimentally Fasciola ‐infected sheep. The data together indicated that the peptides might be of considerable use for discriminating between early and late, and low and high burden, sheep infection with F. gigantica . FasAc14p was chosen to determine whether a peptide based on a critical enzymatic site of cathepsin L proteinase may induce protection against challenge infection. Sheep immunization with FasAc14p peptide induced significant expression of interleukin‐4 mRNA, and humoral antibodies that bound to molecule(s) on the intact surface membrane of newly excysted juvenile worms, and mediated their attrition. The immune responses were associated with significant ( P < 0.02) decrease of 23.1% in worm recovery, but with no decrease in the size or maturation of worms recovered. © 2007 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 90:349–357, 2008. This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com