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Scavenging effects of phenolic compounds on reactive oxygen species
Author(s) -
AboulEnein Hassan Y.,
Kruk Irena,
Kładna Aleksandra,
Lichszteld Krzysztof,
Michalska Teresa
Publication year - 2007
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.20725
Subject(s) - chemistry , singlet oxygen , radical , spin trapping , reactivity (psychology) , hydroxyl radical , reactive oxygen species , superoxide , xanthine oxidase , flavonols , antioxidant , organic chemistry , oxygen , quercetin , medicine , biochemistry , alternative medicine , pathology , enzyme
Phenolic compounds are widely present in plants and they have received considerable attention due to their antioxidant property. In this article we report the results of a study of the reactivity of 10 selected phenolics (sesamol, three phenolic acids, three flavonols, one flavone, and two flavanones) with superoxide anion radical (O 2• –), hydroxyl radical (HO • ) and singlet oxygen ( 1 O 2 ). The following generators of reactive oxygen species were used: 18‐crown‐6/KO 2 /dimethylsulfoxide (DMSO) or hypoxanthine/xanthine oxidase as sources of O 2• –, the Fenton reaction carried out in a sodium trifluoroacetate (pH 6.15) for HO • , and a mixture of alkaline aqueous H 2 O 2 and cobalt ions for 1 O 2 . We have employed chemiluminescence, electron spin resonance spin trapping, and spectrophotometry techniques to examine an antioxidative property. All tested compounds acted as scavengers of various reactive oxygen species. The reactivity indexes (β) for the reaction of the phenolic compounds with HO • were calculated. © 2007 Wiley Periodicals, Inc. Biopolymers 86: 222–230, 2007. This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com