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The interaction between N‐ n ‐undecyl‐N′‐(sodium p ‐aminobenzenesulfonate) thiourea and serum albumin studied using various spectroscopies and the molecular modeling method
Author(s) -
Cui Fengling,
Cui Yanrui,
Luo Hongxia,
Yao Xiaojun,
Fan Jing,
Lu Yan
Publication year - 2006
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.20541
Subject(s) - chemistry , bovine serum albumin , circular dichroism , human serum albumin , thiourea , quenching (fluorescence) , fourier transform infrared spectroscopy , hydrogen bond , fluorescence spectroscopy , fluorescence , spectroscopy , molecule , analytical chemistry (journal) , crystallography , nuclear chemistry , chromatography , organic chemistry , physics , quantum mechanics
The preparation and characteristics of N‐ n ‐undecyl‐N′‐(sodium‐ p ‐aminobenzenesulfonate) thiourea (UPT), a new water‐soluble reagent with a saturated fatty hydrocarbon group, were described. The interactions of UPT with bovine serum albumin (BSA) and human serum albumin (HSA) were studied using fluorescence spectroscopy in combination with ultraviolet (UV) absorption spectroscopy, circular dichroism (CD) spectroscopy, Fourier transform infrared (FTIR) spectroscopy, and the molecular modeling method. UPT exhibited a strong ability to quench the intrinsic fluorescence of both BSA and HSA through a static quenching procedure. The binding constants of UPT and BSA or HSA were determined at different temperatures based on the relevant fluorescence data. The binding sites were obtained and the acting force was suggested to be mainly hydrophobic interaction, which was consistent with the result of the molecular modeling study, and there were also a number of hydrogen bonds between UPT and HSA. The results of determination of the proteins in bovine serum or human serum by this method were very close to those obtained by using Coomassie Brilliant Blue G‐250 colorimetry. A practical method was proposed for the determination of UPT in bovine serum or human serum samples with satisfactory results. © 2006 Wiley Periodicals, Inc. Biopolymers 83: 170–181, 2006 This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com