Premium
Kinetics of the interaction of desAABB–fibrin monomer with immobilized fibrinogen
Author(s) -
Chtcheglova Lilia A.,
Vogel Monique,
Gruber Hermann J.,
Dietler Giovanni,
Haeberli André
Publication year - 2006
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.20529
Subject(s) - fibrinogen , fibrin , chemistry , monomer , surface plasmon resonance , dissociation constant , kinetics , biophysics , stereochemistry , nanotechnology , biochemistry , polymer , organic chemistry , immunology , nanoparticle , receptor , materials science , biology , physics , quantum mechanics
The soluble and stable fibrin monomer–fibrinogen complex (SF) is well known to be present in the circulating blood of healthy individuals and of patients with thrombotic diseases. However, its physiological role is not yet fully understood. To deepen our knowledge about this complex, a method for the quantitative analysis of interaction between soluble fibrin monomers and surface‐immobilized fibrinogen has been established by means of resonant mirror (IAsys) and surface plasmon resonance (BIAcore) biosensors. The protocols have been optimized and validated by choosing appropriate immobilization procedures with regeneration steps and suitable fibrin concentrations. The highly specific binding of fibrin monomers to immobilized fibrin(ogen), or vice versa, was characterized by an affinity constant of ∼10 −8 M , which accords better with the direct dissociation of fibrin triads ( K D ≈ 10 −8 –10 −9 M ) (J. R. Shainoff and B. N. Dardik, Annals of the New York Academy of Science , 1983, Vol. 27, pp. 254–268) than with earlier estimations of the K D for the fibrin–fibrinogen complex ( K D ≈ 10 −6 M ) (J. L. Usero, C. Izquierdo, F. J. Burguillo, M. G. Roig, A. del Arco, and M. A. Herraez, International Journal of Biochemistry , 1981, Vol. 13, pp. 1191–1196). © 2006 Wiley Periodicals, Inc. Biopolymers 83: 69–82, 2006 This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com