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Cellular uptake of phosphorothioate oligonucleotide facilitated by cationic porphyrin: A microfluorescence study
Author(s) -
Kočišová E.,
Praus P.,
Mojzeš P.,
Sureau F.,
Štěpánek J.,
Seksek O.,
Turpin P. Y.
Publication year - 2006
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.20495
Subject(s) - porphyrin , chemistry , oligonucleotide , cationic polymerization , internalization , cytoplasm , fluorescence , rhodamine , biophysics , duplex (building) , dna , photochemistry , cell , biochemistry , polymer chemistry , physics , quantum mechanics , biology
Cationic 5,10,15,20‐tetrakis (1‐methyl‐4‐pyridyl) porphyrin was tested as a delivery agent for oligonucleotides. By using fluorescence microimaging, it has been shown that complexation of the porphyrin to the phosphorothioate analog of dT 15 labeled by rhodamine enabled its nonendocytic penetration into the cell and regular distribution in the cytoplasm and preferentially into the nucleus. Time‐resolved microfluorescence spectroscopy revealed that the oligonucleotide integrity was kept. A small fraction of the porphyrin molecules seems to undergo change of the binding mode after internalization, probably due to duplex formation between the oligonucleotide and its cellular target sequences, or due to dissociation of the porphyrin from the oligonucleotide and subsequent interactions in the cellular environment © 2006 Wiley Periodicals, Inc. Biopolymers 82:325–328, 2006 This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com