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Search for optimal coupling reagent in multiple peptide synthesizer
Author(s) -
Hachmann John,
Lebl Michal
Publication year - 2006
Publication title -
peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.20482
Subject(s) - reagent , preprint , chemistry , combinatorial chemistry , peptide , coupling (piping) , computer science , organic chemistry , biochemistry , world wide web , materials science , metallurgy
Ten different coupling reagents and their combinations were tested in parallel in the synthesis of four model peptide sequences. Significant differences were found between uronium and phosphonium salt‐based reagents and carbodiimide. Diisopropylcarbodiimide was identified as an optimal reagent based on the purity of the product, stability of the reagent, and convenience of handling on plate‐based multiple parallel centrifugation synthesizer. © 2006 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 84: 340–347, 2006 This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com