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Surface‐enhanced Raman scattering study of the interaction of red dye alizarin with ovalbumin
Author(s) -
Cañamares M. V.,
Sevilla P.,
SanchezCortes S.,
GarciaRamos J. V.
Publication year - 2006
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.20450
Subject(s) - alizarin , alizarin red , chemistry , raman spectroscopy , ovalbumin , preprint , organic chemistry , optics , world wide web , staining , physics , pathology , computer science , immune system , immunology , biology , medicine
Surface‐enhanced Raman spectroscopy and UV–vis absorption spectroscopy were employed to study the interaction between the red dye alizarin and ovalbumin (OA), to check the effect of binding media usually employed when applying this pigment in painting practices based on egg tempera. The protein/alizarin interaction is rather weak and takes place through the alizarin neutral form, which interacts with exposed hydrophobic moieties of OA. This effect is of great interest from an artistic point of view because the dye color can be modified. Furthermore, the interaction with alizarin could induce a change in the protein structure, leading to a denaturation and subsequent aggregation. © 2006 Wiley Periodicals, Inc. Biopolymers 82: 405–409, 2006 This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com