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Investigating oligonucleotide hybridization at subnanomolar level by surface plasmon resonance biosensor method
Author(s) -
Vaisocherová Hana,
Zítová Alice,
Lachmanová Markéta,
S̆tĕpánek Josef,
Králíková S̆árka,
Liboska Radek,
Rejman Dominik,
Rosenberg Ivan,
Homola Jir̆í
Publication year - 2006
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.20433
Subject(s) - surface plasmon resonance , chemistry , biosensor , oligonucleotide , streptavidin , nanotechnology , fluorescence , preprint , förster resonance energy transfer , combinatorial chemistry , biotin , nanoparticle , materials science , biochemistry , optics , computer science , physics , dna , world wide web
We have optimized surface plasmon resonance (SPR) biosensor technology for a rapid, direct, and low‐consumption label‐free multianalyte screening of synthetic oligonucleotides (ONs) with modified internucleotide linkages potentially applicable in antisense therapy. Monitoring of the ONs hybridization is based on the formation of complex between the natural oligonucleotide probe immobilized on the sensor surface and the ON in solution in contact with the sensor surface. An immobilization chemistry utilizing the streptavidin–biotin interaction was employed to obtain desired ligand density and high hybridization efficiency. It was demonstrated that the sensor is capable of detecting complementary 23‐mer ONs in concentrations as low as 0.1 n M with high specificity and reproducibility. © 2005 Wiley Periodicals, Inc. Biopolymers 82:394–398, 2006 This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com