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Induction of signaling pathways by herpes simplex virus type 1 through glycoprotein H peptides
Author(s) -
Galdiero Stefania,
Vitiello Mariateresa,
D'Isanto Marina,
Di Niola Erminia,
Peluso Lucia,
Raieta Katia,
Pedone Carlo,
Galdiero Massimiliano,
Benedetti Ettore
Publication year - 2004
Publication title -
peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.20162
Subject(s) - signal transduction , microbiology and biotechnology , mapk/erk pathway , glycoprotein , kinase , protein kinase a , phosphorylation , chemistry , extracellular , mitogen activated protein kinase , herpes simplex virus , biology , biochemistry , virus , virology
Abstract Eukaryotic cells respond to extracellular stimuli, such as viruses, by recruiting signal transduction pathways, many of which are mediated through activation of distinct mitogen‐activated protein kinase (MAPK) cascades and activation of transductional regulation factors. The best characterized of this pathway are the extracellular signal regulated Kinase (ERK), the c‐Jun N‐terminal kinase/stress activated protein kinase (JNK/SAPK), and the p38 MAPK cascade. Herpes simplex virus type 1 (HSV‐1) encodes at least 11 envelope glycoproteins, which alone or in concert play different roles in viral adsorption, entry, cell‐to‐cell spread, and immune evasion. Of these proteins, three are designated glycoprotein B (gB), glycoprotein D (gD), and the gH/gL heterodimer, are clearly involved in attachment and entry, and therefore possible candidates in inducing early cellular activation. Nevertheless, the precise role of each glycoprotein and the cellular factor involved remain elusive. The signal transduction pathways involved, and the outcome of cellular activation on viral entry or postentry events, are still to be elucidated. To better understand the role of signal transduction pathways and phosphorylation events in HSV‐1 entry, synthetic peptides modeled on HSV‐1 gH were synthesized and tested for MEK1‐MEK2/MAPK cascade activation. Our results show a major involvement of the JNK pathway in the intracellular signal transmission after stimulation with gH HSV‐1 peptides. © 2004 Wiley Periodicals, Inc. Biopolymers (Pept Sci), 2004