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Infrared microspectroscopy of individual human cervical cancer (HeLa) cells suspended in growth medium
Author(s) -
Miljkovic Milos,
Romeo Melissa,
Matthäus Christian,
Diem Max
Publication year - 2004
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.20066
Subject(s) - hela , chemistry , infrared , cervical cancer , cell culture , cancer cell , cell , suspension (topology) , cell growth , biophysics , biomedical engineering , cancer , optics , biochemistry , biology , medicine , physics , genetics , mathematics , homotopy , pure mathematics
We report for the first time the infrared spectra of individual human cervical cancer (HeLa) cells suspended in buffer or cell culture medium. Although we did not establish whether these cells were viable at the time of spectral data acquisition, we believe that the methodology used is applicable to the study of live cells. Data were collected either from entire cells, using 25‐ to 40‐μm apertures, or via an imaging approach, where pixels measuring 6.25 × 6.25 μm were assembled to form a map of a cell in suspension. Measurements were carried out both in reflection/absorption and in transmission modes. The results reported here might have far‐reaching implications for the use of infrared microspectroscopy to monitor cell proliferation, drug response, and other cell biological parameters in live cells. © 2004 Wiley Periodicals, Inc. Biopolymers, 2004