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The biological potency of a series of analogues of human calcitonin correlates with their interactions with phospholipids
Author(s) -
Epand Raquel F.,
Orlowski Ronald C.,
Epand Richard M.
Publication year - 2004
Publication title -
peptide science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.20030
Subject(s) - chemistry , potency , calcitonin , series (stratigraphy) , stereochemistry , pharmacology , biochemistry , medicine , in vitro , paleontology , biology
The conformational and lipid binding properties of several calcitonin analogs were compared. These analogs were designed to have the central amphipathic helical region of salmon calcitonin and N‐ and C‐terminal segments similar to human calcitonin. The various analogs differed from one another either by removal of Leu 19 from this hybrid analog, replacement of Leu 19 with Gly 19 or having a carboxyl terminus more closely related to salmon calcitonin. It had been found that replacement of Leu 19 with Gly 19 caused a marked reduction in the hypocalemic activity of the analog. The ability of the analogs to form helical structures in the presence of dimyristoylphosphatidylglycerol as well as their ability to lower the enthalpy of the calorimetric phase transition of this phospholipid correlates well with the hypocalcemic potency of the peptide. © 2004 Wiley Periodicals, Inc. Biopolymers (Pept Sci), 2004