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Strategies for covalent attachment of DNA to beads
Author(s) -
Steinberg Gali,
Stromsborg Katie,
Thomas Lynette,
Barker David,
Zhao Chanfeng
Publication year - 2004
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.20006
Subject(s) - cyanuric chloride , chemistry , covalent bond , dna , chloroformate , linker , hydrazone , dna–dna hybridization , combinatorial chemistry , isothiocyanate , organic chemistry , biochemistry , computer science , operating system
Several covalent attachment chemistries were tested for the immobilization of DNA onto glass beads. The comparison was based on the ability of these chemistries to produce derivatized beads that give good hybridization signals. Cyanuric chloride, isothiocyanate, nitrophenyl chloroformate, and hydrazone chemistries gave us the best (yet comparable) hybridization signals. We further characterized the cyanuric chloride method for the number of attachment sites, number of hybridizable sites, hybridization kinetics, effect of linker length on hybridization intensity and stability of the derivatized beads. © 2004 Wiley Periodicals, Inc. Biopolymers, 2004