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Effects of some organic cosolvents on the functional properties of hemoglobin: Kinetics of O 2 displacement by CO
Author(s) -
Cordone Lorenzo,
Cupane Antonio
Publication year - 1981
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1981.360201008
Subject(s) - chemistry , formamide , acetamide , kinetics , hemoglobin , methanol , ethanol , equilibrium constant , propanol , medicinal chemistry , organic chemistry , inorganic chemistry , physics , quantum mechanics
We studied the kinetics of replacement of O 2 by CO in hemoglobin in the presence and absence of organic cosolvents (methanol, ethanol, iso ‐propanol, n ‐propanol, formamide, acetamide, N ‐methyl‐formamide) and at 10 and 25°C. Quantitative analysis of the results indicates that these cosolvents do not affect the intrinsic binding constants of ligands to the heme when hemoglobin is in the R conformation. The present results confirm the previously reported suggestion that the effects of the above cosolvents on the oxygen affinity of hemoglobin are related to effects on the T ⇄ R conformational equilibrium.