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β‐Turn preference of tetrapeptide sequences as analyzed by CD spectra of their Dnp‐pNA derivatives
Author(s) -
Sato K.,
Kawai M.,
Nagai U.
Publication year - 1981
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1981.360200915
Subject(s) - tetrapeptide , chemistry , sign (mathematics) , turn (biochemistry) , preference , spectral line , chirality (physics) , circular dichroism , exciton , cotton effect , stereochemistry , crystallography , series (stratigraphy) , wavelength , peptide , optics , physics , chiral symmetry , mathematics , mathematical analysis , condensed matter physics , quantum mechanics , biochemistry , statistics , paleontology , nambu–jona lasinio model , biology , quark
The Dnp‐pNA derivatives of more than 30 tetrapeptides were synthesized and subjected to CD measurements in MeOH. The CD spectra were analyzed on the basis of exciton chirality theory and were found to be useful for estimating β‐turn preference of the parent tetrapeptides based on the following facts: (1) when a compound of the series takes the βturn conformation, a pair of strong CD bands with opposite sign are observed at 305 and 350 nm, and (2) when it is in a random or nonfolded conformation, no significant bands are observed at the wavelengths. The intensity of the 350‐nm band reflects especially well the βturn preference. The CD spectra can be classified into two groups according to the sign of the band at 350 nm, and the groups seem to correspond to the type of βturn. By summarizing the results obtained, some generalizations concerning the sequence–conformation relationship are proposed.