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Infrared study of the secondary structure of lac repressor and its tryptic core
Author(s) -
Schnarr Manfred,
Maurizot JeanClaude
Publication year - 1980
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1980.360191104
Subject(s) - lac repressor , chemistry , repressor , lac operon , core protein , core (optical fiber) , infrared spectroscopy , biochemistry , gene , escherichia coli , organic chemistry , transcription factor , materials science , composite material
The lac repressor and its tryptic core were studied by ir spectroscopy, and their β‐structure content was determined by analysis of the spectra. Using protein‐derived reference spectra, we find a β‐content for lac repressor of 18% and of 23% for its tryptic core. The higher amount of β‐conformation in the tryptic core is confirmed by another type of analysis (decomposition of the spectra in Gaussian curves). These results are discussed with respect to their implications for the structure of the N‐terminal “headpiece” of lac repressor and for the mode of interaction of lac repressor with lac operator.