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Rigidity of fibrin gels as measured by quasielastic light scattering
Author(s) -
Gelman R. A.,
Gladner J. A.,
Nossal R.
Publication year - 1980
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1980.360190702
Subject(s) - chemistry , fibrin , shear modulus , light scattering , rigidity (electromagnetism) , fibrinogen , modulus , electrophoresis , polyacrylamide , swelling , polymer chemistry , scattering , composite material , chromatography , materials science , optics , biochemistry , physics , immunology , biology
Abstract The strength of fibrin gels has been investigated by a recently developed laser light scattering technique for determining the shear modulus of soft gels. By this method, changes in the modulus were monitored as a function of time without perturbing the material. Fibrin gels were crosslinked with blood coagulation factor XIII. Rigidity measurements and SDS–polyacrylamide gel electrophoresis were used to correlate gel strength with the number of covalently bonded subunit chains. The modulus was found to vary linearly with the number of crosslinks until maximum rigidity was achieved.