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Measurement of preferential solvation of some glucans in mixed solvent systems by gel‐permeation chromatography
Author(s) -
Straub Peggy R.,
Brant David A.
Publication year - 1980
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1980.360190313
Subject(s) - chemistry , solvation , solvent , dimethyl sulfoxide , solvation shell , permeation , chromatography , organic chemistry , membrane , biochemistry
Preferential solvation of the glucans amylose, pullulan, and dextran in binary dimethyl‐sulfoxide/water (DMSO/H 2 O) solvent mixtures has been measured using gel‐permeation chromatography. The preferential solvation behavior of the three glucans in DMSO/H 2 O solvent mixtures is indistinguishable in the experiments reported. In solvent mixtures with mol ratio DMSO/H 2 O less than 1:2, all three glucans are solvated preferentially by H 2 O. The maximum extent of preferential solvation by H 2 O is about 2.5 mol H 2 O/mol of glucose residues. When the DMSO/H 2 O mol ratio exceeds 1:2, DMSO solvates the glucans preferentially to a maximum extent of about 1 mol DMSO/mol of glucose residues. An interpretation of the change in preferential solvation with mixed solvent composition is suggested in terms of the known characteristics of the binary solvent system, and the relationship of preferential solvation, reported here, to the absolute solvation of the glucan chains is discussed.