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Formation of ternary complexes involving zinc or copper ions, polynucleotides, and polypeptides containing glutamic acid and tyrosine residues
Author(s) -
Bere Augustin,
Helene Claude
Publication year - 1979
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1979.360181103
Subject(s) - polynucleotide , chemistry , nucleic acid , ternary operation , tyrosine , zinc , metal ions in aqueous solution , fluorescence , copper , crystallography , quenching (fluorescence) , stereochemistry , metal , biochemistry , organic chemistry , physics , quantum mechanics , computer science , programming language
Metal ions such as Zn 2+ and Cu 2+ can mediate interactions between copolypeptides (Glu x , Tyr y ) n and polynucleotides. CD data show that these ternary complexes are characterized by an unstacking of nucleic acid bases, while the polypeptide adopts an α‐helical conformation as observed in the two binary complexes polynucleotide–cation and polypeptide–cation. Fluorescence studies demonstrate that tyrosyl side chains interact with nucleic acid bases in the ternary complexes, leading to a quenching of tyrosine fluorescence.