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Dynamic light scattering from collagen solutions. II. Photon correlation study of the depolarized light
Author(s) -
Thomas John C.,
Fletcher Guy C.
Publication year - 1979
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1979.360180603
Subject(s) - chemistry , dynamic light scattering , rotational diffusion , autocorrelation , extrapolation , monomer , diffusion , light scattering , spectroscopy , analytical chemistry (journal) , correlation function (quantum field theory) , photon , molecular physics , component (thermodynamics) , scattering , thermodynamics , optics , chromatography , physics , polymer , mathematical analysis , statistics , mathematics , optoelectronics , organic chemistry , quantum mechanics , molecule , nanoparticle , dielectric
The depolarized forward‐scattered light from solutions of rat tail collagen has been studied by photon correlation spectroscopy. The measured autocorrelation function is seen to decay on two widely different time scales. The decay time for the fast component is consistent with the rotational diffusion of rodlike collagen monomers. The slowly decaying autocorrelation component is attributed to large nonspecific aggregates of collagen. A substantial fraction of the collagen is in this aggregated form. Extrapolation of the faster decay times to zero concentration yields a value of θ 20,w 0= 1082 ± 30 sec −1 for the rotational diffusion coefficient of the collagen monomer.