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Changes in the refractive index ellipsoid isotropies, symmetric anisotropies, and depolarization ratios of potassium hyaluronate solutions as a function of pH
Author(s) -
Barrett T. W.
Publication year - 1979
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1979.360180211
Subject(s) - chemistry , refractive index , anisotropy , isotropy , dipole , polarization (electrochemistry) , biopolymer , ellipsoid , condensed matter physics , analytical chemistry (journal) , optics , chromatography , polymer , organic chemistry , physics , astronomy
A new method is presented for studying the tertiary and possibly the secondary structure of a biopolymer, which is applied to the pH‐dependent structural transition of potassium hyaluronate solutions. The method involves a new application of Dirac dispersion theory polarization dependence measures to isotropic and anisotropic components of the refractive index ellipsoid. Changes in the dipole moment may also be observed by this method. The pH‐induced changes in viscosity and optical properties of hyaluronic acid previously reported appear to be dependent on changes in secondary and tertiary structure.