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Density‐gradient‐sedimentation velocity of solvated macromolecules: Theoretical considerations about the buoyancy factor
Author(s) -
Nieuwenhuysen Paul
Publication year - 1979
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1979.360180205
Subject(s) - solvation , chemistry , sedimentation , density gradient , buoyancy , sedimentation coefficient , macromolecule , context (archaeology) , chemical physics , implicit solvation , velocity gradient , thermodynamics , physics , geology , molecule , sediment , organic chemistry , geomorphology , paleontology , biochemistry , quantum mechanics , enzyme
The solvation of macromolecules lowers their sedimentation velocity in a density gradient. Some consequences of this effect are pointed out. Overlooking the solvation results in the understimation of the standard sedimentation coefficient of the macromolecules. (The error increases with partial specific volume, solvation, and the density in the gradient.) The construction of an isokinetic density gradient also requires that solvation be taken into account. This can be done when the solvation parameter, relevant in this context, is known.