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Biosynthèse de cellulose bactérienne à pertir de glycérol sélectivement deutérié en position 1, 2, ou 3: Etude par résonance magnétique nucléaire
Author(s) -
ChumpitaziHermoza Bernabé F.,
Gagnaire Didier,
Taravel François R.
Publication year - 1978
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.1978.360171006
Subject(s) - cellulose , chemistry , glycerol , deuterium , bacterial cellulose , nuclear chemistry , polymer chemistry , organic chemistry , physics , nuclear physics
Glycerol specifically deuterated at C‐1, C‐2, or C‐3 was prepared and used for the biosynthesis of bacterial cellulose with Acetobacter xylinum. The material obtained were converted into glucitol hexaacetate and analyzed by 250‐MHz nuclear magnetic resonance and mass spectrometry. The spectra indicated that the protons of the C‐3 position of the starting glycerol were incorporated as substituents of the C‐6 and C‐1 positions of the cellulose. Similarly, protons of the C‐2 and C‐5 positions of the cellulose came essentially from water and the protons bonded at the C‐3 and C‐4 positions of the cellulose from protons bonded to C‐1 of the starting glycerol.